Research interests


Our current research is focused on the mechanisms and biological relevance of genome maintenance processes.  The emphasis is on homologous DNA recombination because this is a versatile process that can assist in the repair of a variety of lesions, such as DNA double-strand breaks, interstrand DNA crosslinks and stalled DNA replication forks.  We are pursuing an integrated approach using whatever technique is most relevant to address the particular mechanistic question at hand.  We are using biochemical, protein structural, cell biological and mouse genetics methods to unravel different aspect of homologous recombination from the molecular to the whole animal level (see Project pages).  We are developing reagents and equipment to analyze the molecular interactions between the many proteins required for homologous recombination at the single molecule level.  Our approaches include scanning force microscopy, magnetic tweezers for DNA manipulation and total internal reflection microscopy.  Furthermore, genome maintenance pathways are analyzed in living cells using confocal video-microscopy and photo-bleaching techniques.  From our initial studies it has become clear that there is extensive crosstalk among genome surveillance pathways.  Therefore, our future studies will include a number of other genome surveillance pathways in addition to homologous recombination.  Our studies in living cells have also directed our attention to the interplay between recombination and DNA replication.  Mouse genetics approaches using both knock-in and knock-out strategies to generate endogenously expressed fluorescent fusion proteins of interest and mice with deficiencies in multiple genome surveillance pathways are also actively pursued.  This research line will address the biological effects of genome maintenance pathway crosstalk and produce appropriate cells for the analysis of genome maintenance mechanisms in living cells. 

 
Roland Kanaar studied chemistry at Leiden University and obtained his PhD degree in 1988 for research on the action of an enhancer in site-specific DNA recombination and the elucidation of how nucleoprotein complexes assembled at distant sites along a DNA chain communicate with each other to provide selectivity during recombination. His post-doctoral work with at the University of California, Berkeley, aimed at understanding mechanisms of homologous recombination (with Nick Cozzarelli) and at understanding how proteins and RNA interact to achieve accurate but flexible recognition of splice sites (with Don Rio). His current
research addresses the mechanisms and biological relevance of genome surveillance processes with particular emphasis on homologous DNA recombination and DNA double-strand break repair. Genome surveillance is essential to prevent chromosomal abnormalities, which in their turn may lead to hereditary diseases, cancer or cell decay. In 2000 he was appointed Professor of Molecular Radiation Genetics. He is a scientific co-founder of the biotechnology company DNage, which is focused on the development of products for medical and health problems associated with ageing. He serves on the scientific advisory board of the FIRC Institute of Molecular Oncology Foundation in Milan, Italy, and on the editorial boards of a number of scientific journals including EMBO Journal and Molecular Cell. In 2002 he was elected as a member of the European Molecular Biology Organization.
 

Publications


  

   127   van Loenhout, M.T., van der Heijden, T., Kanaar, R., Wyman, C. and Dekker, C.  (2009). Dynamics of RecA filaments on single-stranded DNA.  Nucleic Acids Res., in press.


   126   Warmerdam, D.O., Freire, R., Kanaar, R. and Smits, V.A.  (2009).  Cell cycle-dependent processing of DNA lesions controls localization of Rad9 to sites of genotoxic stress.  Cell Cycle 8, 1765-1774.


   125   van der Linden, H.P., Sanchez, H., Kinoshita, E., Kanaar, R. and Wyman, C.  (2009).  RAD50 and NBS1 form a stable complex functional in DNA binding and tethering.  Nucleic Acids Res. 37, 1580-1588


   124   van Mameren, J., Modesti, M., Kanaar, R., Wyman, C., Peterman, E. J. and Wuite, G. J.  (2009).  Counting RAD51 proteins disassembling from nucleoprotein filaments under tension.  Nature 457, 745-748.


   123   Kirshner, M., Finkel, M., Nizan, A., Essers, J., Kanaar, R., Shiloh, Y. and Barzilai, A.  (2009).  Analysis of the relationships between ATM and the Rad54 Paralogs involved in homologous recombination repair.  DNA Repair 8, 253-261.


   122   Budzowska. M. and Kanaar, R.  (2009).  Mechanisms of dealing with DNA damage-induced replication problems.  Cell Biochem. Biophys. 53, 17-31.


   121   Medhurst, A. L., Warmerdam, D. O., Akerman, I., Verwayen, E. H., Kanaar, R., Smits, V. A. and Lakin, N. D.  (2008). ATR and Rad17 collaborate in modulating Rad9 localisation at sites of DNA damage.  J. Cell Sci. 121, 3393-3949.


   120   Krawczyk, P. M., Stap, J., Hoebe, R. A., van Oven, C. H., Kanaar, R. and Aten, J. A.  (2008).  Analysis of the mobility of DNA double-strand break-containing chromosome domains in living mammalian cells.  Methods Mol. Biol. 463, 309-320.


   119   Kanaar, R. and Wyman, C.  (2008).  DNA repair by the MRN complex:  Break it to make it.  Cell 135, 14-16.


   118   Van der Heijden, T., Modesti, M., Hage, S., Kanaar, R., Wyman, C., Dekker, C.  (2008).  Homologous recombination in real time: DNA strand exchange by RecA.  Mol. Cell 30, 530-538. 


   117    Wyman, C., Warmerdam, D. O. and Kanaar, R.  (2008).  From DNA end chemistry to cell-cycle response:  The importance of structure, even when it’s broken. Mol. Cell 30, 5-6.


   116   Kanaar, R., Wyman, C. and Rothstein, R.  (2008).  Quality control in DNA break metabolism:  In the ‘end’, it’s a good thing.  EMBO J. 27, 581-588.


   115   Stap, J., Krawczyk, P.M., van Oven, C.H., Barendsen, G., Essers, J., Kanaar, R. and Aten, J.A.  (2008).  Induction of linear tracks of DNA double-strand breaks by a-particle irradiation of cells. Nature Meth. 5, 261-266. 


   114   Modesti, M., Budzowska, M., Baldeyron, C., Demmers, J.A., Ghirlando, R. and Kanaar, R. (2007).  RAD51AP1 is a structure-specific DNA binding protein that stimulates joint molecule formation during RAD51-mediated homologous recombination.  Mol. Cell 28, 468-481.


   113   Marchetti, F., Essers, J., Kanaar, R. and Wyrobek, A.J.  (2007).  Ionizing radiation-induced sperm DNA lesions are repaired in the egg by the NHEJ and HR pathways.  Proc. Natl. Acad. Sci. USA 104, 17725-17729.


   112   Hanada, K., Budzowska, M., Davies, S.L., van Drunen, E., Beverloo, B.H., Maas, A., Essers, J., Hickson, I.D. and Kanaar, R.  (2007).  Involvement of the structure-specific endonuclease Mus81-Eme1 in double-strand DNA break-dependent replication restart.  Nat. Struct. Mol. Biol. 14, 1096-1104.


   111   Van der Heijden, T., Seidel, R., Modesti, M., Kanaar, R., Wyman, C., Dekker, C. (2007).  Real-time assembly and disassembly of human RAD51 filaments on individual DNA molecules.  Nucleic Acids Res. 35, 5646-5657.


   110   Dinant, C., de Jager, M., Essers, J., van Cappellen, W.A., Kanaar, R., Houtsmuller, A.B. and Vermeulen, W.  (2007).  Activation of multiple dna repair pathways by sub-nuclear damage induction methods.  J. Cell Sci. 120, 2731-2740.


   109   Agarwal, S., Kanaar, R. and Essers, J.  (2007).  The cell biology of homologous recombination.  In ‘Molecular Genetics of Recombination’, Aguilera, A. and Rothstein, R. (Eds.), Topics Curr. Genet. (Springer Verlag), Germany, 335-362.


   108   Heijden, T.V., Moreno-Herrero, F., Kanaar, R., Wyman, C. and Dekker, C.  (2007).  AFM Tip-Induced Dissociation of RecA-dsDNA Filaments.  Nano Lett. 7, 1112.


   107   Modesti, M., Ristic, D., van der Heijden, T. Dekker, C., van Mameren, J., Peterman, E.J., Wuite, G.J., Kanaar, R. and Wyman, C.  (2007).  Fluorescent human Rad51 reveals multiple nucleation sites and filament segments tightly associated along a single DNA molecule.  Structure 15, 599-609.


   106   Hanada, K.,  Vermeij, M., Garinis, G.A., de Waard, M.C., Kunen, M.G.S., Myers, L., Maas, A., Duncker, D.J., Meijers, C., Dietz, H.C., Kanaar, R. and Essers, J.  (2007).  Perturbations of vascular homeostasis and aortic valve abnormalities in Fibulin-4 deficient mice.  Circulation Res. 100, 738-746.


   105   Brugmans, L., Kanaar, R. and Essers, J. (2007).  Analysis of DNA double-strand break repair pathways in mice.  Mutat. Res. 614, 95-108.


   104   Heijden, T.V., Moreno-Herrero, F., Kanaar, R., Wyman, C. and Dekker, C.  (2006).  Comment on "Direct and real-time visualization of the disassembly of a single RecA-DNA-ATPgS Complex using AFM imaging in fluid".  Nano Lett. 6, 3000-3002.


   103   Rakhorst, H.A., Tra, W.M.W., Posthumus-van Sluijs, S.T., Houtgraaf, J., Levendag P.C., Kanaar, R. and Hofer, S.O.P.  (2006).  A quantitative model for radiation-induced DNA break repair in cultured oral mucosal substitutes.  Tissue Eng. 12, 3395-3403.


   102   Hanada, K., Budzowska, M., Modesti, M., Maas, A., Wyman, C., Essers, J. and Kanaar, R.  (2006).  The structure-specific endonuclease Mus81-Eme1 promotes conversion of interstrand DNA cross-links into double-strands breaks.  EMBO J. 25, 4921-4932.


   101   Mohd-Sarip, A., van der Knaap, J.A., Wyman, C., Kanaar, R. , Schedl, P. and Verrijzer, C.P.  (2006).  Architecture of a Polycomb nucleoprotein complex.  Mol. Cell 24, 91-100.


   100   van Mameren, J., Modesti, M., Kanaar, R., Wyman, C., Wuite, G.J. and Peterman, E.J.  (2006).  Dissecting elastic heterogeneity along DNA molecules coated partly with Rad51 using concurrent fluorescence microscopy and optical tweezers.  Biophys. J. 91, L78-80.


   99   Wyman, C. and Kanaar, R.  (2006).  DNA double-strand break repair:  All’s well that ends well.  Ann. Rev. Genet. 40, 363-383. 


   98   Eppink, B., Wyman, C. and Kanaar, R.  (2006).  Multiple interlinked mechanisms to circumvent DNA replication roadblocks.  Exp. Cell Res. 312, 2660-2665.


   97   Agarwal, S., Tafel, A.A. and Kanaar, R.  (2006).  DNA double-strand break repair and chromosomal translocations.  DNA Repair 5, 1075-1081. 


   96   Essers, J., Houtsmuller, A.B. and Kanaar, R.  (2006).  Analysis of DNA recombination and repair proteins in living cells by photobleaching microscopy.  Meth. Enzymol. 408, 463-485.


   95   Aten, J.A. and Kanaar, R.  (2006).  Chromosomal Organization:  Mingling with the neighbors.  PLoS Biol. 4, 689-691.


   94   Kanaar, R. and Sherratt, D.  (2006).  Nicholas (Nick) R. Cozzarelli (1938-2006).  Cell 125, 415-417.


   93   Wesoly, J., Agarwal, S., Sigurdsson, S., Bussen, W., Van Komen, S., Qin, J., Van Steeg, H., Van Benthem, J., Wassenaar, E., Baarends, W.M., Ghazvini, M., Tafel, A.A., Heath, H., Galjart, N., Essers, J., Grootegoed, J.A., Arnheim, N., Bezzubova, O., Buerstedde, J.M., Sung, P. and Kanaar, R.  (2006).  Differential contributions of mammalian Rad54 paralogs to recombination, DNA damage repair and meiosis.  Mol. Cell. Biol. 26, 976-989. 


   92   Van Veelen, L.R., Wesoly, J. and Kanaar, R.  (2006).  Biochemical and cellular aspects of homologous recombination.  In DNA Damage Recognition, W. Siede, Y.W. Kow, and P.W. Doetsch, eds., Taylor & Francis Group, New York, pp. 581-607.


   91   Garinis, G.A., Mitchell, J.R., Moorhouse, M.J., Hanada, K., de Waard, H., Vandeputte, D., Jans, J., Brand, K., Smid, M., van der Spek, P.J., Hoeijmakers, J.H., Kanaar, R. and van der Horst, G.T.  (2005).  Transcriptome analysis reveals cyclobutane pyrimidine dimers as major source of UV-induced DNA breaks.  EMBO J. 24, 3952-3962.


   90   Essers, J., Theil, A.F., Baldeyron, C., Van Cappellen, W.A., Houtsmuller, A.B., Kanaar, R., Vermeulen, W.  (2005).  Nuclear dynamics of PCNA in DNA replication and repair.  Mol. Cell. Biol. 21, 9350-9359. 


   89   Moreno-Herrero, F., de Jager, M., Dekker, N.H., Kanaar, R., Wyman, C. and Dekker, C.  (2005).  Mesoscale conformational changes in the DNA repair complex Rad50/Mre11/Nbs1 upon DNA binding.  Nature 437, 440-443.


   88   Ristic, D., Modesti, M., van der Heijden, T., van Noort, J., Dekker, C., Kanaar, R. and Wyman, C.  (2005).  Human Rad51 filaments on double- and single-stranded DNA:  Correlating regular and irregular forms with recombination function. Nucleic Acids Res. 33, 3292-3302.


   87   Van Veelen, L.R., Essers, J., van de Rakt, M.W., Paulusma, C.C. and Kanaar, R.  (2005).  Ionizing radiation-induced foci formation of mammalian Rad51 and Rad54 proteins depends on the Rad51 paralogs, but not on Rad52.  Mutat. Res. 574, 34-49.


   86   Van Veelen, L.R., Cervelli, T., van de Rakt, M.W, Theil, A.F., Essers, J. and Kanaar, R.  (2005).  Analysis of ionizing radiation-induced foci of DNA damage repair proteins.  Mutat. Res. 574, 22-33.


   85   van der Heijden, T., van Noort, J., van Leest, H, Kanaar, R., Wyman, C., Dekker, N. and Dekker, C.  (2005). Torque-limited RecA polymerization on double-stranded DNA.  Nucleic Acids Res. 33, 2099-2105.


   84   Essers, J., van Cappellen, W.A., Theil, A.F., van Drunen, E., Jaspers, N.G., Hoeijmakers, J.H., Wyman, C., Vermeulen, W. and Kanaar, R.  (2005).  Dynamics of relative chromosome position during the cell cycle.  Mol. Biol. Cell 16, 769-775.


   83   Beerens, N., Hoeijmakers, J.H., Kanaar, R., Vermeulen, W. and Wyman, C.  (2005).  The CSB protein actively wraps DNA.  J. Biol. Chem. 280, 4722-4729. 


   82   Adams, D.J., van der Weyden, L., Gergely, F.V., Arends, M.J., Ng, B.L., Tannahill, D., Kanaar, R., Markus, A., Morris, B.J. and Bradley, A. (2005).  BRCTx is a novel, highly conserved RAD18-interacting protein.  Mol. Cell. Biol. 25, 779-788. 


   81   Van Veelen, L.R., Kanaar, R. and van Gent, D.C.  (2005).  DNA repair and malignant hematopoiesis.  In Textbook of Malignant Hematology (2nd Edition), L. Degos, D.C. Linch, and B. Löwenberg, eds., Taylor & Francis Group, London, UK, pp. 155-164.


   80   Heyer, W.-D. and Kanaar, R.  (2004).  Recombination mechanisms:  Fortieth anniversary of the Holliday model.  Mol. Cell 16, 1-9. 


   79   Budzowska, M., Jaspers, I., Essers, J., De Waard, H., Van Drunen, E., Hanada, K., Beverloo, B., Hendriks, R.W., De Klein, A., Kanaar, R., Hoeijmakers, J.H. and Maas, A.  (2004).  Mutation of the mouse Rad17 gene leads to embryonic lethality and reveals a role in DNA damage-dependent recombination.  EMBO J. 23, 3548-3558.


   78   Kanaar, R.  (2004).  Mechanisms and biologilca relevance of DNA damage repair.  Daniel den Hoed Cancer News 3, 2-5. 


   77   Lau, A., Kanaar, R., Jackson, S.P., O'Connor, M.J.  (2004).  Suppression of retroviral infection by the RAD52 DNA repair protein.  EMBO J. 23, 3421-3429.


   76   Wyman, C. and Kanaar, R.  (2004).  Homologous recombination:  Down to the wire.  Curr. Biol. 14, R629-631.


   75   Wyman, C., Ristic, D. and Kanaar, R.  (2004).  Homologous recombination-mediated double-strand break repair.  DNA Repair 3, 827-833.


   74   McPherson, J.P., Lemmers, B., Chahwan, R., Pamidi, A., Migon, E., Matysiak-Zablocki, E., Moynahan, M.E., Essers, J., Hanada, K., Poonepalli, A., Sanchez-Sweatman, O., Khokha, R., Kanaar, R., Jasin, M., Hande, M.P. and Hakem, R.  (2004).  Involvement of mammalian Mus81 in genome integrity and tumor suppression.  Science 304, 1822-1826. 


   73   Niedernhofer, L.J., Odijk, H., Budzowska, M., van Drunen, E., Maas, A., Theil, A.F., de Wit, J., Jaspers, N.G.J., Beverloo, H.B., Hoeijmakers, J.H.J. and Kanaar, R.  (2004).  The structure-specific endonuclease Ercc1-Xpf is required to resolve DNA interstrand crosslink-induced double-strand breaks.  Mol. Cell. Biol. 24, 5776-5787.


   72   Couedel, C., Mills, K.D., Barchi, M., Shen, L., Oishen, A., Johnson, R.D., Nussenzweig, A., Essers, J., Kanaar, R., Li, G.C., Alt, F.W. and Jasin, M.  (2004).  Collaboration of homologous recombination and nonhomologous end-joining factors to the survival and integrity of mice and cells.  Genes Dev. 18, 1293-1304.


   71   Mills, K.D., Ferguson, D.O., Essers, J., Eckersdorff, M., Kanaar, R. and Alt, F.W.  (2004).  Rad54 and DNA Ligase IV cooperate to maintain mammalian chromatid stability.  Genes Dev. 18, 1283-1292. 


   70   de Jager, M., Trujillo, K.M., Sung, P., Hopfner, K.P., Carney, J.P., Tainer, J.A., Connelly, J.C., Leach, D.F.R., Kanaar, R. and Wyman, C.  (2004).  Differential arrangements of conserved building blocks among homologs of the Rad50/Mre11 DNA repair protein complex.  J. Mol. Biol. 339, 937-949.


   69   Aten, J.A., Stap, J., Krawczyk, P.M., van Oven, C.H., Hoebe, R.A., Essers, J. and Kanaar, R.  (2004).  Dynamics of DNA double-strand breaks revealed by clustering of damaged chromosome domains.  Science 303, 92-95. 


   68   Abraham, J., Lemmers, B., Hande, M.P., Moynahan, M.E. , Chahwan, C., Ciccia, A., Essers, J., Hanada, K., Chahwan, R., Khaw, A. K., McPherson, P., Shehabeldin, A., Laister, R., Arrowsmith, C., Kanaar, R., West, S.C., Jasin, M. and Hakem, R.  (2003).  Eme1 is involved in DNA damage processing and maintenance of genomic stability in mammalian cells.  EMBO J. 22, 6137-6147.


   67   Modesti, M. Junop, M.S., Ghirlando, R., Van der Rakt, M., Gellert, M., Yang, W. and Kanaar, R.  (2003).  Tetramerization and DNA ligase IV interaction of the DNA double-strand break repair protein XRCC4 are mutually exclusive.  J. Mol. Biol. 334, 215-228.


   66   Ristic, D., Modesti. M., Kanaar, R. and Wyman, C.  (2003).  Rad52 and Ku bind to different DNA structures produced early in double-strand break repair.  Nucleic Acids Res. 31, 5229-5237. 


   65   Tan, T.L.R., Kanaar, R. and Wyman, C.  (2003).  Rad54, a Jack of all trades in homologous recombination.  DNA Repair 2, 787-794. 


   64   Van Noort, J., van der Heijden, T., de Jager, M., Wyman, C., Kanaar, R. and Dekker, C.  (2003).  The coiled-coil of the human Rad50 DNA repair protein contains specific segments of increased flexibility.  Proc. Natl. Acad. Sci. USA 100, 7581-7586.


   63   Savouret, C., Brisson, E., Essers, J., Kanaar, R., Pastink, A., te Riele, H., Junien, C. and Gourdon, G.  (2003).  CTG repeat instability and size variation timing in DNA repair-deficient mice.  EMBO J. 22, 2264-2273. 


   62   Bross, L., Wesoly, J., Buerstedde, J.M., Kanaar, R. and Jacobs, H.  (2003).  Somatic hypermutation does not require Rad54 and Rad54B-mediated homologous recombination.  Eur. J. Immunol. 33, 352-357.


   61   De Jager, M., Wyman, C., van Gent, D.C. and Kanaar, R.  (2002).  DNA end-binding specificity of human Rad50/Mre11 is influenced by ATP.  Nucleic Acids Res. 30, 4425-4431.


   60   Essers, J., Hendriks, R.W., Wesoly, J., Beerens, C.E.M.T., Smit, B., Hoeijmakers, J.H.J., Wyman, C., Dronkert, M.L.G. and Kanaar, R.  (2002).  Analysis of mouse Rad54 expression and its implications for homologous recombination.  DNA Repair 1, 779-793. 


   59   D'Avirro, N., Truomg, D., Luong, M., Kanaar, R. and Selsing, E.  (2002).  Gene conversion-like sequence transfers between transgenic antibody V genes are independent of Rad54.  J. Immunol. 169, 3069-3075. 


   58   De Jager, M. and Kanaar, R.  (2002).  Genome instability and Rad50S: Subtle yet severe.  Genes Dev. 16, 2173-2178. 


   57   Difilippantonio, M.J., Petersen, S., Chen, H.T., Johnson, R., Jasin, M., Kanaar, R., Ried, T. and Nussenzweig, A.  (2002).  Evidence for replicative repair of DNA double-strand breaks leading to oncogenic translocation and gene amplification.  J. Exp. Med. 196, 469-480. 


   56   Wyman, C. and Kanaar, R.  (2002).  Chromosome organization: Reaching out to embrace new models.  Curr. Biol. 12, R446-448.


   55   Godthelp, B.C., Wiegant, W.W., Van Duijn-Goedhart, A., Schärer, O.D., Van Buul, P.P.W., Kanaar, R. and Zdzienicka, M.Z.  (2002).  Mammalian Rad51C contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability.  Nucleic Acid Res. 30, 2172-2182. 


   54   Essers, J., Houtsmuller, A.B., van Veelen, L., Paulusma, C., Nigg, A.L., Pastink, A., Vermeulen, W., Hoeijmakers, J.H.J. and Kanaar, R.  (2002).  Nuclear dynamics of RAD52 group homologous recombination proteins in response to DNA damage.  EMBO J. 21, 2030-2037. 


   53   Kraakman-van der Zwet, M., Overkamp, W.J.I., van Lange, R.E.E., Essers, J., van Duijn-Goedhart, A., Wiggers, I., Swaminathan, S.,  van Buul, P.P.W., Errami, A., Tan, R.T.L., Jaspers, N.G.J., Sharan, S.K., Kanaar, R. and Zdzienicka, M.Z.  (2002).  Brca2 (XRCC11) deficiency results in radioresistant DNA synthesis and a higher frequency of spontaneous deletions.  Mol. Cell. Biol. 22, 669-679.


   52   De Jager, M., van Noort, J., van Gent, D.C., Dekker, C., Kanaar, R. and Wyman, C.  (2001).  Human Rad50/Mre11 is a flexible complex that can tether DNA ends.  Mol. Cell 8, 1129-1135.


   51   Niedernhofer, L.J., Essers, J., Weeda, G., Beverloo, H.B., Muitjens, M., Odijk, H., de Wit, J., Hoeijmakers, J.H.J. and Kanaar, R.  (2001).  The structure-specific endonuclease Ercc1/Xpf is required for targeted gene replacement in embryonic stem cells.  EMBO J. 22, 6540-6549. 


   50   Dronkert, M.L.G. and Kanaar, R.  (2001).  Repair of DNA interstand crosslinks.  Mutat. Res. 486, 217-247. 


   49   Loonstra, A., Vooijs, M., Beverloo, H.B., Allak, B.A., van Drunen, E., Kanaar, R., Berns, A. and Jonkers, J.  (2001).  Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells.  Proc. Natl. Acad. Sci. USA 98, 9209-9214. 


   48   Ristic, D., Wyman, C., Paulusma, C. and Kanaar, R.  (2001).  The architecture of the human Rad54-DNA complex provides evidence for protein translocation along DNA.  Proc. Natl. Acad. Sci. USA 98, 8454-8460. 


   47   Modesti, M. and Kanaar, R.  (2001).  Homologous recombination: From model organisms to human disease.  Genome Biol., 2, 1014.1-1014.5. 


   46   Modesti, M. and Kanaar, R.  (2001).  DNA repair: Spot(light)s on chromatin.  Curr. Biol. 11, R229-R232. 


   45   Van Gent, D.C., Hoeijmakers, J.H.J. and Kanaar, R.  (2001).  Chromosomal stability and the DNA double-stranded break connection.  Nat. Rev. Genet. 2, 196-206.


   44   De Jager, M., Dronkert. M.L.G., Modesti, M., Beerens, C.E.M.T., Kanaar, R. and van Gent, D.C.  (2001).  DNA binding and strand annealing activities of human Mre11:  Implications for its roles in DNA double-strand break repair pathways.  Nucleic Acids Res. 29,1317-1325. 


   43   Kanaar, R.  (2000).  DNA damage:  The rise and demise of a cancer cell.  South West Cancer News 1, 7. 


   42   Citterio, E., Schnitzler, G., van den Boom, V., Kanaar, R., Bonte, E., Kingston, R.E., Hoeijmakers, J.H.J. and Vermeulen, W.  (2000).  ATP-dependent chromatin remodeling by the Cockayne syndrome B DNA repair-transcription-coupling factor.  Mol. Cell. Biol. 20, 7643-7653.


   41   Takata, M., Sasaki, M.S., Sonoda, E., Fukushima, T., Morrison, C., Albala, J.S., Swagemakers, S.M.A., Kanaar, R., Thompson, L.H. and Takeda, S.  (2000).  The Rad51 paralog Rad51B promotes homologous recombinational repair.  Mol. Cell. Biol. 20, 6476-6482. 


   40   Dronkert, M.L.G., de Wit, J., Boeve, M., Vasconcelos, M.L., van Steeg, H., Tan, T.L.R., Hoeijmakers, J.H.J. and Kanaar, R.  (2000).  Disruption of mouse SNM1 causes increased sensitivity to the DNA interstrand cross-linking agent mitomycin C.  Mol. Cell. Biol. 20, 4553-4561. 


   39   Dronkert, M.L.G., Beverloo, H.B., Johnson, R.D., Hoeijmakers, J.H.J., Jasin, M. and Kanaar, R.  (2000).  Mouse RAD54 affects DNA double-strand break repair and sister chromatid exchange.  Mol. Cell. Biol. 20, 3147-3156. 


   38   Essers, J., van Steeg, H., de Wit, J., Swagemakers, S.M.A., Vermeij, M., Hoeijmakers, J.H.J. and Kanaar, R.  (2000).  Homologous and non-homologous recombination differentially affect DNA damage repair in mice.  EMBO J. 19, 1703-1710. 


   37   Bennink, M.L, Schärer, O.D., Kanaar, R., Sokata-Sogawa, K., Schins, J.M., Kanger, J.S., de Grooth, B.G. and Greve, J.  (1999).  Single molecule manipulation of double-stranded DNA using optical tweezers: interaction studies of DNA with RecA and YOYO-1.  Cytometry 36, 200-208. 


   36   Parisi, S., McKay, M.J., Molnar, M., Thompson, M.A., van der Spek, P.J.. van Drunen-Schoenmaker, E., Kanaar, R., Lehmann, E., Hoeijmakers, J.H.J. and Kohli, J.  (1999).  Rec8p, a meiotic recombination and sister chromatid cohesion phosphoprotein of the Rad21p family conserved from fission yeast to humans. Mol. Cell. Biol. 19, 3515-3528. 


   35   Crowder, S.M., Kanaar, R., Rio, D.C. and Alber, T.  (1999).  Absence of interdomain contacts in the crystal structure of the RNA recognition motifs of Sex-lethal.  Proc. Natl. Acad. Sci. USA 96, 4892-4897.


   34   Tan, T.L.R., Essers, J., Citterio, E., Swagemakers, S.M.A., de Wit, J., Benson, F.E., Hoeijmakers, J.H.J. and Kanaar, R.  (1999).  Mouse Rad54 affects DNA conformation and DNA-damage-induced Rad51 foci formation.  Curr. Biol. 9, 325-328. 


   33   Kanaar, R.  (1999).  Molecular mechanisms of ionizing radiation-induced DNA damage repair.  South West Cancer News 4, 5-6.


   32   Kanaar, R. (1999).  Molecular mechanisms of DNA damage repair after irradiation.  In Vascular Brachytherapy:  New Perspectives (P.C. Levendag, Ed.), Remedica Publishing Ltd, London, pp 17-21. 


   31   Van den Berg, A.P. and Kanaar, R.  (1999).  Repair mechanisms of radiation-induced DNA damage.  South West Cancer News 2, 5-8. 


   30   Kanaar, R., Hoeijmakers, J.H.J. and van Gent, D.C.  (1998).  Molecular mechanisms of DNA double-strand break repair.  Trends Cell Biol. 8, 483-489.


   29   Swagemakers, S.M.A., Essers, J., de Wit, J., Hoeijmakers, J.H.J. and Kanaar, R.  (1998).  The human Rad54 recombinational DNA repair protein is a double-stranded DNA-dependent ATPase.  J. Biol. Chem. 273, 28292-28297. 


   28   Rudner, D.Z, Breger, K.S., Kanaar, R., Adams, M.D. and Rio, D.C.  (1998).  RNA binding of heterodimeric splicing factor U2AF:  at least one RS domain is required for high-affinity binding.  Mol. Cell. Biol. 18, 4004-4011. 


   27   Rudner, D.Z, Kanaar, R., Breger, K.S. and Rio, D.C.  (1998).  Interaction between subunits of the heterodimeric splicing factor U2AF is essential in vivo.  Mol. Cell. Biol. 18, 1765-1773. 


   26   Kanaar, R. and Hoeijmakers, J.H.J.  (1998).  From competition to collaboration.  Nature 391, 335-338.


   25   Hammond, L.E., Rudner, D.Z., Kanaar, R. and Rio, D.C.  (1997).  Mutations in the hrp48 gene, which encodes a Drosophila heterogeneous nuclear ribonucleoprotein particle protein, cause lethality and developmental defects and affect P element third-intron splicing in vivo.  Mol. Cell. Biol. 17, 7260-7267. 


   24   Lee, A.L., Volkman, B.F., Robertson, S.A., Rudner, D.Z., Barbash, D.A., Cline, T.W., Kanaar, R., Rio, D.C. and Wemmer, D.E.  (1997).  Chemical shift mapping of the RNA-binding interface of the multiple-RBD protein Sex-lethal.  Biochemistry 36, 14306-14317. 


   23   Kanaar R. and Hoeijmakers, J.H.J.  (1997).  Recombination and joining; Different means to the same ends.  Genes Funct. 1, 165-174. 


   22   Essers, J., Hendriks, R.W., Swagemakers, S.M.A., Troelstra, C., de Wit, J., Bootsma, D., Hoeijmakers, J.H.J. and Kanaar, R.  (1997).  Disruption of mouse RAD54 reduces ionizing radiation resistance and homologous recombination.  Cell 89, 195-204. 


   21   Lehmann, A.R., Bridges, B.A., Hanawalt, P.C., Johnson, R.T., Kanaar, R., Krokan, H.E., Kyrtopoulos, S., Lambert, B., Melton, D.W., Moustacchi, E., Natarajan, A.T., Radman, M., Sarasin, A., Seeberg, E., Smerdon, M.J., Smith, C.A., Smith, P.J., Thacker, J., Thomale, J., Waters, R., Weeda, G., West, S.C., van Zeeland, A.A. and Zdzienicka, M.Z.  (1996).  Workshop on processing of DNA damage.  Mutat. Res. 364, 245-270. 


   20   Rudner, D.Z., Kanaar, R., Breger, K.S. and Rio, D.C.  (1996).  Mutations in the small subunit of the Drosophila U2AF splicing factor cause lethality and developmental defects.  Proc. Natl. Acad. Sci. USA 93, 10333-10337. 


   19   McKay, M.J., Troelstra, C., Van der Spek, P., Kanaar, R., Smit, B., Hagemeijer, A., Bootsma, D. and Hoeijmakers, J.H.J.  (1996).  Sequence conservation of the rad21 Schizosaccharomyces pombe DNA double-strand break repair gene in human and mouse.  Genomics 36, 305-315. 


   18   Kanaar, R., Troelstra, C., Swagemakers, S., Essers, J., Smit, B., Franssen, J.-H., Pastink, A., Bezzubova, O., Buerstedde, J.-M., Clever, B., Heyer, W.-D. and Hoeijmakers, J.H.J.  (1996).  Human and mouse homologs of the Saccharomyces cerevisiae RAD54 DNA repair gene:  Evidence for functional conservation.  Curr. Biol. 6, 828-838. 


   17   Benjamin, K, Abola, P., Kanaar, R. and Cozzarelli, N.R.  (1996).  Contributions of supercoiling to resolvase and Gin recombination.  J. Mol. Biol. 256, 50-65. 


   16   Kanaar, R., Lee, A.L., Rudner, D.Z., Wemmer, D.E. and Rio, D.C.  (1995).  Interaction of the Sex-lethal RNA binding domains with RNA.  EMBO J. 14, 4530-4539. 


   15   Lee, A.L., Kanaar, R., Rio, D.C. and Wemmer, D.E.  (1994).  Resonance assignments and solution structure of the second RNA-binding domain of Sex-lethal determined by multidimensional heteronuclear magnetic resonance.  Biochemistry 33, 13775-13786. 


   14   Crisona, N.J., Kanaar, R, Gonzalez, T.N., Zechiedrich, E.L., Klippel, A. and Cozzarelli, N.R.  (1994).  Processive recombination by wild-type Gin and an enhancer-independent mutant:  Insight into the mechanisms of recombination selectivity and strand exchange.  J. Mol. Biol. 243, 437-457.


   13   Siebel, C.W., Kanaar, R. and Rio, D.C.  (1994).  Regulation of tissue-specific P element pre-mRNA splicing requires the RNA binding protein PSI.  Genes Dev. 8, 1713-1725.


   12   Chen, J., Kanaar, R. and Cozzarelli, N.R.  (1994).  The SEP1 strand exchange protein from Saccharomyces cerevisiae promotes a paranemic joint between homologous DNA molecules.  Genes Dev. 8, 1356-1366.


   11   Kanaar, R., Roche, S.E., Beall, E.L., Green, M.R. and Rio, D.C.  (1993).  The conserved pre-mRNA splicing factor U2AF from Drosophila:  Requirement for viability.  Science 262, 569-573.


   10   Klippel, A., Kanaar, R., Kahmann, R. and Cozzarelli, N.R.  (1993).  Analysis of strand exchange and DNA binding of enhancer-independent Gin recombinase mutants.  EMBO J. 12, 1047-1059.


   9   Kanaar, R. and Cozzarelli, N.R.  (1992).  Roles of supercoiled DNA structure in DNA transactions.  Curr. Opin. Struct. Biol. 2, 369-379.


   8   Kanaar, R., Klippel, A., Shekhtman, E., Dungan, J.M., Kahmann, R. and Cozzarelli, N.R.  (1990).  Processive recombination by the phage Mu Gin system:  Implications for mechanisms of DNA strand exchange, DNA site alignment, and enhancer action.  Cell 62, 353-366.


   7   Kanaar, R., Van Hal, J.P. and Van de Putte, P.  (1989).  The recombinational enhancer for DNA inversion functions independent of its orientation as a consequence of dyad symmetry in the Fis-DNA complex.  Nucleic Acids Res. 17, 6043-6053.


   6   Kanaar, R., Van de Putte, P. and Cozzarelli, N.R.  (1989).  Gin-mediated recombination of catenated and knotted DNA substrates:  Implications for the mechanism of interaction between cis-acting sites.  Cell 58, 147-159.


   5   Kanaar, R., Van de Putte, P. and Cozzarelli, N.R.  (1988).  Gin-mediated DNA inversion:  Product structure and the mechanism of strand exchange.  Proc. Natl. Acad. Sci. USA 85, 752-756.


   4   Koch, C., Mertens, G., Rudt, F., Kahmann, R., Kanaar, R., Plasterk, R.H.A., Van de Putte, P., Sandulache, R. and Kamp, D.  (1987).  The invertible G segment.  In Phage Mu, N. Symonds, A. Toussaint, P. Van de Putte and M.M. Howe, eds., Cold Spring Harbor, New York:  Cold Spring Harbor Laboratory, pp. 75-91.


   3   Kanaar, R. and Van de Putte, P.  (1987).  Topological aspects of site-specific DNA inversion.  BioEssays 7, 195-200.


   2   Kanaar, R., Van de Putte, P. and Cozzarelli, N.R.  (1986).  Purification of Gin recombination protein of Escherichia coli phage Mu and its host factor.  Biochim. Biophys. Acta 166, 170-177.


   1   Plasterk, R.H.A., Kanaar, R. and Van de Putte, P.  (1984).  A genetic switch in vitro:  DNA inversion by Gin   

                protein of phage Mu.  Proc. Natl. Acad. Sci. USA 81, 2689-2692. 






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