DNA replication restart

We are interested in the link between DNA replication and recombination. Homologous recombination is essential for DNA replication, because it is required to restart stalled replication. We analyze eukaryotic structure-specific endonucleases, such as Mus81, that resolve DNA structures generated in response to stalled replication (either before and/or after the action of homologous recombination enzymes). We apply techniques such as pulsed field gel electrophoresis and chromosomal fiber analyses.

Colocalization of the homologous recombination protein Rad51 (green) with sites of stalled DNA replication (red).

See Hanada, et al.  (2007).  Involvement of the structure-specific endonuclease Mus81-Eme1 in double-strand DNA break-dependent replication restart.  Nat. Struct. Mol. Biol. 14, 1096-1104.

Analysis of replication recovery on chromosomal fibers using two differentialy labeled nucleoside analogs.